The main ingredient in Botox 100IU Injection is botulinum toxin type A. It is being used more and more to treat spasms and other nerve problems. A bacteria called Clostridium botulinum is used to make it. Botox 100IU Injection is injected deep into the skin or into the muscles that need it to fix wrinkles, fine lines, and stiff muscles. It makes the muscles briefly loose, which makes the forehead and eyes wrinkle.